Anti-STAT4 Polyclonal Antibody (HC549014)

Name: Anti-STAT4 Polyclonal Antibody
Brand: abinScience
SKU: HC549014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC549014

Description

Anti-STAT4 Polyclonal Antibody (HC549014) is a rabbit polyclonal antibody detecting STAT4 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STAT4 (Tyr505-Ile737).

Target

Signal transducer and activator of transcription 4, STAT4

Purification

Purified by antigen affinity column.

Accession

Q14765

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Signal transducer and activator of transcription 4 (STAT4) is a ~85 kDa protein. Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes.

1. Visconti, R. et al. (2000) Blood 96, 1844-52. PMID: 10961885

2. Baghdassarian, H. et al. (2023) The New England journal of medicine 388, 2241-2252. PMID: 37256972

3. Cho, SS. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 4781-9. PMID: 8943379

4. Morinobu, A. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 12281-6. PMID: 12213961

5. Zhang, YS. et al. (2022) Cell death and differentiation 29, 2303-2315. PMID: 35614130

6. Yao, BB. et al. (1999) Archives of biochemistry and biophysics 368, 147-55. PMID: 10415122

7. Bacon, CM. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7307-11. PMID: 7638186

8. Oppmann, B. et al. (2000) Immunity 13, 715-25. PMID: 11114383

9. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746

10. Gonzales-van Horn, SR. et al. (2016) European journal of immunology 46, 1504-10. PMID: 26990433

Note

For research use only.

Images

References

Datasheet

Anti-STAT4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-STAT4 Polyclonal Antibody (HC549014)

Overview

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References

Datasheet

SDS

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