Anti-SETDB1 Polyclonal Antibody (HC332014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

HC332014

Description

Anti-SETDB1 Polyclonal Antibody (HC332014) is a rabbit polyclonal antibody detecting SETDB1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SETDB1 (Met1-Ser350).

Target

H3-K9-HMTase 4, ESET, SETDB1, SET domain bifurcated 1, Histone H3-K9 methyltransferase 4, KMT1E, KIAA0067, Lysine N-methyltransferase 1E, ERG-associated protein with SET domain, Histone-lysine N-methyltransferase SETDB1

Purification

Purified by antigen affinity column.

Accession

Q15047

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone-lysine N-methyltransferase SETDB1 is a ~143 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.

1. Schultz, DC. et al. (2002) Genes & development 16, 919-32. PMID: 11959841

2. Ayyanathan, K. et al. (2003) Genes & development 17, 1855-69. PMID: 12869583

3. Wang, H. et al. (2003) Molecular cell 12, 475-87. PMID: 14536086

4. Sun, L. et al. (2016) Molecular cell 62, 958-966. PMID: 27237050

5. Chang, YH. et al. (2024) Cell reports 43, 114536. PMID: 39096901

6. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

7. Timms, RT. et al. (2016) Cell reports 17, 653-659. PMID: 27732843

9. Valle-García, D. et al. (2016) Epigenetics 11, 398-414. PMID: 27029610

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SETDB1 polyclonal antibody at 1ug/mL on recombinant SETDB1 (Catalog No. HC332012).

References

Datasheet

Anti-SETDB1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SETDB1 Polyclonal Antibody (HC332014)

Overview

Images

References

Datasheet

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