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Anti-OCLN Polyclonal Antibody (HS920014)

Anti-OCLN Polyclonal Antibody
Anti-OCLN Polyclonal Antibody
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  • 50ug
  • 100ug
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Overview
Catalog No.HS920014
Description
Anti-OCLN Polyclonal Antibody (HS920014) is a rabbit polyclonal antibody detecting OCLN in ELISA, IHC, WB, IF. Suitable for Human, Mouse, Dog, Rat, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Dog, Rat, Pongo abelii
ApplicationsELISA, IHC, WB, IF
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human OCLN (Lys266-Thr522).
Target Occludin, OCLN
Purification Purified by antigen affinity column.
Accession Q16625
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Occludin (OCLN) is a ~59 kDa protein. May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

Note For research use only.
Images
  • Anti-OCLN Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-OCLN polyclonal antibody at 1ug/mL on recombinant OCLN (Catalog No. HS920012).

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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