Anti-RSPO1 Polyclonal Antibody (HS709014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HS709014

Description

Anti-RSPO1 Polyclonal Antibody (HS709014) is a rabbit polyclonal antibody detecting RSPO1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RSPO1 (Ser21-Ala135).

Target

R-spondin-1, hRspo1, RSPO1, Roof plate-specific spondin-1

Purification

Purified by antigen affinity column.

Accession

Q2MKA7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

R-spondin-1 (RSPO1) is a ~28 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.

1. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720

2. Binnerts, ME. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 14700-5. PMID: 17804805

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RSPO1 polyclonal antibody at 1ug/mL on recombinant RSPO1 (Catalog No. HS709012).

References

Datasheet

Anti-RSPO1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RSPO1 Polyclonal Antibody (HS709014)

Overview

Images

References

Datasheet

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