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Anti-ADIPOR2 Polyclonal Antibody (HV258014)

Anti-ADIPOR2 Polyclonal Antibody
Anti-ADIPOR2 Polyclonal Antibody
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Overview
Catalog No.HV258014
Description
Anti-ADIPOR2 Polyclonal Antibody (HV258014) is a rabbit polyclonal antibody detecting ADIPOR2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ADIPOR2 (Met1-Gly147).
Target Adiponectin receptor protein 2, ADIPOR2, PAQR2, Progestin and adipoQ receptor family member 2, Progestin and adipoQ receptor family member II
Purification Purified by antigen affinity column.
Accession Q86V24
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adiponectin receptor protein 2 (ADIPOR2) is a ~43 kDa protein. Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels.

1. Yamauchi, T. et al. (2003) Nature 423, 762-9. PMID: 12802337
2. Tanabe, H. et al. (2015) Nature 520, 312-316. PMID: 25855295
Note For research use only.
Images
  • Anti-ADIPOR2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ADIPOR2 polyclonal antibody at 1ug/mL on recombinant ADIPOR2 (Catalog No. HV258012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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