Anti-CD177 Polyclonal Antibody (HV109014)

Name: Anti-CD177 Polyclonal Antibody
Brand: abinScience
SKU: HV109014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV109014

Description

Anti-CD177 Polyclonal Antibody (HV109014) is a rabbit polyclonal antibody detecting CD177 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD177 (Cys133-Ser301).

Target

CD177, NB1 GP, HNA-2a, Human neutrophil alloantigen 2a, NB1, PRV-1, CD177 antigen, Polycythemia rubra vera protein 1, NB1 glycoprotein, PRV1

Purification

Purified by antigen affinity column.

Accession

Q8N6Q3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD177 antigen is a ~46 kDa protein. In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF primed neutrophils including degranulation and superoxide production. In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration. Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro. However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo. Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils.

1. Jerke, U. et al. (2011) The Journal of biological chemistry 286, 7070-81. PMID: 21193407

2. Bai, M. et al. (2017) Blood 130, 2092-2100. PMID: 28807980

3. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308

4. Wang, L. et al. (2013) European journal of haematology 90, 436-7. PMID: 23461681

5. von Vietinghoff, S. et al. (2007) Blood 109, 4487-93. PMID: 17244676

6. von Vietinghoff, S. et al. (2008) Clinical and experimental immunology 152, 508-16. PMID: 18462208

7. Kuckleburg, CJ. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 275-84. PMID: 23202369

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD177 polyclonal antibody at 1ug/mL on recombinant CD177 (Catalog No. HV109012).

References

Datasheet

Anti-CD177 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD177 Polyclonal Antibody (HV109014)

Overview

Images

References

Datasheet

SDS

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