Anti-DDB2 Polyclonal Antibody (HV031014)

Name: Anti-DDB2 Polyclonal Antibody
Brand: abinScience
SKU: HV031014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HV031014

Description

Anti-DDB2 Polyclonal Antibody (HV031014) is a rabbit polyclonal antibody detecting DDB2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DDB2 (Pro166-Met383).

Target

Damage-specific DNA-binding protein 2, DNA damage-binding protein 2, UV-damaged DNA-binding protein 2, DDB2, UV-DDB 2, DDBb, DDB p48 subunit

Purification

Purified by antigen affinity column.

Accession

Q92466

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA damage-binding protein 2 (DDB2) is a ~47 kDa protein. Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage.

1. Tang, JY. et al. (2000) Molecular cell 5, 737-44. PMID: 10882109

2. Wakasugi, M. et al. (2001) The Journal of biological chemistry 276, 15434-40. PMID: 11278856

3. Wakasugi, M. et al. (2002) The Journal of biological chemistry 277, 1637-40. PMID: 11705987

4. Groisman, R. et al. (2003) Cell 113, 357-67. PMID: 12732143

5. Sugasawa, K. et al. (2005) Cell 121, 387-400. PMID: 15882621

6. Kapetanaki, MG. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2588-93. PMID: 16473935

7. Guerrero-Santoro, J. et al. (2008) Cancer research 68, 5014-22. PMID: 18593899

8. Geng, A. et al. (2020) Nucleic acids research 48, 9181-9194. PMID: 32789493

9. Hwang, BJ. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 424-8. PMID: 9892649

Note

For research use only.

Images

References

Datasheet

Anti-DDB2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-DDB2 Polyclonal Antibody (HV031014)

Overview

Images

References

Datasheet

SDS

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