Anti-TSLP Polyclonal Antibody (HV243014)

Name: Anti-TSLP Polyclonal Antibody
Brand: abinScience
SKU: HV243014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV243014

Description

Anti-TSLP Polyclonal Antibody (HV243014) is a rabbit polyclonal antibody detecting TSLP in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TSLP (Tyr29-Gln159).

Target

TSLP, Thymic stromal lymphopoietin

Purification

Purified by antigen affinity column.

Accession

Q969D9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Thymic stromal lymphopoietin (TSLP) is a ~18 kDa protein. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting TSLP (HV243014) at a concentration of 11.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of TSLP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet