Anti-P2RX7 Polyclonal Antibody (HV713014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HV713014

Description

Anti-P2RX7 Polyclonal Antibody (HV713014) is a rabbit polyclonal antibody detecting P2RX7 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human P2RX7 (Asp356-Tyr595).

Target

P2RX7, Purinergic receptor, P2X purinoceptor 7, P2X7, P2Z receptor, ATP receptor

Purification

Purified by antigen affinity column.

Accession

Q99572

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

P2X purinoceptor 7 (P2RX7) is a ~68 kDa protein. ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP.

1. Riedel, T. et al. (2007) Biophysical journal 93, 846-58. PMID: 17483156

2. Robinson, LE. et al. (2014) The Journal of biological chemistry 289, 31983-31994. PMID: 25281740

3. Rassendren, F. et al. (1997) The Journal of biological chemistry 272, 5482-6. PMID: 9038151

4. Adinolfi, E. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 3393-404. PMID: 20453110

5. Pippel, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2156-E2165. PMID: 28235784

6. Markwardt, F. et al. (2024) PNAS nexus 3, pgae349. PMID: 39262850

7. Mou, YJ. et al. (2024) Biochemical and biophysical research communications 737, 150500. PMID: 39142135

8. Karmakar, M. et al. (2016) Nature communications 7, 10555. PMID: 26877061

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-P2RX7 polyclonal antibody at 1ug/mL on recombinant P2RX7 (Catalog No. HV713012).

References

Datasheet

Anti-P2RX7 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-P2RX7 Polyclonal Antibody (HV713014)

Overview

Images

References

Datasheet

Quick access

Contact Us