Anti-NUP85 Polyclonal Antibody (HV235014)

Name: Anti-NUP85 Polyclonal Antibody
Brand: abinScience
SKU: HV235014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV235014

Description

Anti-NUP85 Polyclonal Antibody (HV235014) is a rabbit polyclonal antibody detecting NUP85 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NUP85 (Arg130-Leu368).

Target

Pericentrin-1, Nucleoporin Nup85, Nucleoporin Nup75, PCNT1, 85 kDa nucleoporin, NUP75, NUP85, Nuclear pore complex protein Nup85, FROUNT

Purification

Purified by antigen affinity column.

Accession

Q9BW27

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nuclear pore complex protein Nup85 is a ~75 kDa protein. Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.

1. Harel, A. et al. (2003) Molecular cell 11, 853-64. PMID: 12718872

2. Orjalo, AV. et al. (2006) Molecular biology of the cell 17, 3806-18. PMID: 16807356

3. Terashima, Y. et al. (2005) Nature immunology 6, 827-35. PMID: 15995708

4. Braun, DA. et al. (2018) The Journal of clinical investigation 128, 4313-4328. PMID: 30179222

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NUP85 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HT-29 cell lysate
Lane 3: LO2 cell lysate
Lane 4: 293T cell lysate

References

Datasheet

Anti-NUP85 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-NUP85 Polyclonal Antibody (HV235014)

Overview

Images

References

Datasheet

SDS

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