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Anti-HIF1AN Polyclonal Antibody (HV428014)

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Overview
Catalog No.HV428014
Description
Anti-HIF1AN Polyclonal Antibody (HV428014) is a rabbit polyclonal antibody detecting HIF1AN in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HIF1AN (Ala2-Asn349).
Target Factor inhibiting HIF-1, FIH1, FIH-1, HIF1AN, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase
Purification Purified by antigen affinity column.
Accession Q9NWT6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) is a ~40 kDa protein. Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively.

Note For research use only.
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Datasheet
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Formula
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-HIF1AN Polyclonal Antibody

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