Anti-NOD1 Polyclonal Antibody (HV077014)

Name: Anti-NOD1 Polyclonal Antibody
Brand: abinScience
SKU: HV077014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV077014

Description

Anti-NOD1 Polyclonal Antibody (HV077014) is a rabbit polyclonal antibody detecting NOD1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NOD1 (Ala612-Asp826).

Target

NOD1, Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, CARD4

Purification

Purified by antigen affinity column.

Accession

Q9Y239

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a ~107 kDa protein. Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling.

1. Inohara, N. et al. (2001) The Journal of biological chemistry 276, 2551-4. PMID: 11058605

2. Girardin, SE. et al. (2003) Science (New York, N.Y.) 300, 1584-7. PMID: 12791997

3. Chamaillard, M. et al. (2003) Nature immunology 4, 702-7. PMID: 12796777

4. Tanabe, T. et al. (2004) The EMBO journal 23, 1587-97. PMID: 15044951

5. Girardin, SE. et al. (2005) The Journal of biological chemistry 280, 38648-56. PMID: 16172124

6. Fukazawa, A. et al. (2008) PLoS pathogens 4, e1000228. PMID: 19043560

7. Lautz, K. et al. (2012) Cellular microbiology 14, 1568-83. PMID: 22672233

8. Vegna, S. et al. (2016) Journal of virology 90, 6022-6035. PMID: 27099311

10. Magalhaes, JG. et al. (2005) EMBO reports 6, 1201-7. PMID: 16211083

Note

For research use only.

Images

References

Datasheet

Anti-NOD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-NOD1 Polyclonal Antibody (HV077014)

Overview

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References

Datasheet

SDS

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