Anti-Bovine DNTT/TDT Polyclonal Antibody (BY380014)

Name: Anti-Bovine DNTT/TDT Polyclonal Antibody
Brand: abinScience
SKU: BY380014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

BY380014

Description

Anti-Bovine DNTT/TDT Polyclonal Antibody (BY380014) is a rabbit polyclonal antibody detecting DNTT in ELISA, IHC, WB. Suitable for Human, Bovine, and Eulemur macaco.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bovine

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Bovine DNTT/TDT (Phe139-Ala509).

Target

DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT

Purification

Purified by antigen affinity column.

Accession

P06526

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA nucleotidylexotransferase (DNTT/TDT) is a ~58 kDa protein. Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

1. Koiwai, O. et al. (1986) Nucleic acids research 14, 5777-92. PMID: 3755527

Note

For research use only.

Images

References

Datasheet

Anti-Bovine DNTT/TDT Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Bovine DNTT/TDT Polyclonal Antibody (BY380014)

Overview

Images

References

Datasheet

SDS

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