Anti-Human ATG4A Polyclonal Antibody (HP478014)

Name: Anti-Human ATG4A Polyclonal Antibody
Brand: abinScience
SKU: HP478014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP478014

Description

Anti-Human ATG4A Polyclonal Antibody (HP478014) is a rabbit polyclonal antibody detecting ATG4A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATG4A (Met1-Val398).

Target

hAPG4A, Cysteine protease ATG4A, ATG4A, AUTL2, Autophagin-2, APG4A, AUT-like 2 cysteine endopeptidase, Autophagy-related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2

Purification

Purified by antigen affinity column.

Accession

Q8WYN0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cysteine protease ATG4A is a ~45 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Scherz-Shouval, R. et al. (2003) The Journal of biological chemistry 278, 14053-8. PMID: 12473658

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

5. Shu, CW. et al. (2011) Journal of biomolecular screening 16, 174-82. PMID: 21245471

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Nguyen, N. et al. (2020) The Journal of biological chemistry 295, 13584-13600. PMID: 32732290

9. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106

10. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288

Note

For research use only.

Images

References

Datasheet

Anti-Human ATG4A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human ATG4A Polyclonal Antibody (HP478014)

Overview

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Datasheet

SDS

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