Anti-Human DDX39B/BAT1/UAP56 Polyclonal Antibody (HC553014)

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100ug

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Datasheet

Overview

Catalog No.

HC553014

Description

Anti-Human DDX39B/BAT1/UAP56 Polyclonal Antibody (HC553014) is a rabbit polyclonal antibody detecting DDX39B in ELISA, IHC, WB. Suitable for Bovine, Dog, Chicken, Human, and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Dog, Drosophila melanogaster, Chicken, Human, Macaca mulatta, Mouse, Pan troglodytes, Pongo abelii, Rat, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DDX39B/BAT1/UAP56 (Leu259-Arg428).

Target

BAT1, Spliceosome RNA helicase DDX39B, ATP-dependent RNA helicase p47, DEAD box protein UAP56, DDX39B, UAP56, HLA-B-associated transcript 1 protein, 56 kDa U2AF65-associated protein

Purification

Purified by antigen affinity column.

Accession

Q13838

RRID

Anti-Human DDX39B/BAT1/UAP56 Polyclonal Antibody (abinScience Cat# HC553014, RRID:AB_3730431)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Spliceosome RNA helicase DDX39B (BAT1/UAP56) is a ~48 kDa protein. Involved in nuclear export of spliced and unspliced mRNA. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. The TREX complex is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway. The THOC1-THOC2-THOC3 core complex alone is sufficient to promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B. Associates with SARNP/CIP29, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export.

1. Guo, S. et al. (2005) Cancer research 65, 3011-6. PMID: 15833825

2. Masuda, S. et al. (2005) Genes & development 19, 1512-7. PMID: 15998806

3. Cheng, H. et al. (2006) Cell 127, 1389-400. PMID: 17190602

4. Pühringer, T. et al. (2020) eLife 9. PMID: 33191911

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human DDX39B/BAT1/UAP56 polyclonal antibody at 1ug/mL on recombinant Human DDX39B/BAT1/UAP56 (Catalog No. HC553012).

References

Datasheet

Anti-Human DDX39B/BAT1/UAP56 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human DDX39B/BAT1/UAP56 Polyclonal Antibody (HC553014)

Overview

Images

References

Datasheet

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