Anti-Human CBS Polyclonal Antibody (HW451014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HW451014

Description

Anti-Human CBS Polyclonal Antibody (HW451014) is a rabbit polyclonal antibody detecting CBS in ELISA, IHC, WB. Suitable for Human, Mouse, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Macaca fascicularis, Oryctolagus cuniculus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CBS (Lys72-Lys551).

Target

Serine sulfhydrase, CBS, Cystathionine beta-synthase, Beta-thionase

Purification

Purified by antigen affinity column.

Accession

P35520

RRID

Anti-Human CBS Polyclonal Antibody (abinScience Cat# HW451014, RRID:AB_3730475)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cystathionine beta-synthase (CBS) is a ~60 kDa protein. Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.

1. Kozich, V. et al. (2010) Human mutation 31, 809-19. PMID: 20506325

2. Mendes, MI. et al. (2014) Journal of inherited metabolic disease 37, 245-54. PMID: 23974653

3. Casique, L. et al. (2013) Gene 531, 117-24. PMID: 23981774

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CBS polyclonal antibody at 1ug/mL on recombinant Human CBS (Catalog No. HW451012).

References

Datasheet

Anti-Human CBS Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CBS Polyclonal Antibody (HW451014)

Overview

Images

References

Datasheet

Quick access

Contact Us