Anti-Human ATG7 Polyclonal Antibody (HF840014)

Name: Anti-Human ATG7 Polyclonal Antibody
Brand: abinScience
SKU: HF840014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HF840014

Description

Anti-Human ATG7 Polyclonal Antibody (HF840014) is a rabbit polyclonal antibody detecting ATG7 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATG7 (Ser19-Glu321).

Target

hAGP7, ATG7, Ubiquitin-activating enzyme E1-like protein, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, Autophagy-related protein 7, ATG12-activating enzyme E1 ATG7, APG7-like

Purification

Purified by antigen affinity column.

Accession

O95352

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin-like modifier-activating enzyme ATG7 is a ~77 kDa protein. E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes.

1. Collier, JJ. et al. (2021) The New England journal of medicine 384, 2406-2417. PMID: 34161705

Note

For research use only.

Images

References

Datasheet

Anti-Human ATG7 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATG7 Polyclonal Antibody (HF840014)

Overview

Images

References

Datasheet

SDS

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