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Anti-Human PEX14 Polyclonal Antibody (HF863014)

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Overview
Catalog No.HF863014
Description
Anti-Human PEX14 Polyclonal Antibody (HF863014) is a rabbit polyclonal antibody detecting PEX14 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PEX14 (Arg25-Glu76).
Target Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, PEX14, Peroxisomal membrane anchor protein PEX14
Purification Purified by antigen affinity column.
Accession O75381
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Peroxisomal membrane protein PEX14 is a ~41 kDa protein. Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix. Plays a key role for peroxisome movement through a direct interaction with tubulin.

1. Neuhaus, A. et al. (2014) The Journal of biological chemistry 289, 437-48. PMID: 24235149
2. Dias, AF. et al. (2017) The Journal of biological chemistry 292, 15287-15300. PMID: 28765278
3. Fransen, M. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 8087-92. PMID: 9653144
4. Bharti, P. et al. (2011) Journal of cell science 124, 1759-68. PMID: 21525035
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human PEX14 Polyclonal Antibody

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