Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Name: Anti-Human ATP6V1A Polyclonal Antibody
Brand: abinScience
SKU: HW292014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW292014

Description

Anti-Human ATP6V1A Polyclonal Antibody (HW292014) is a rabbit polyclonal antibody detecting ATP6V1A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATP6V1A (Lys456-Asp617).

Target

ATP6V1A1, ATP6A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69 kDa subunit, V-ATPase subunit A, ATP6V1A, Vacuolar proton pump subunit alpha, Vacuolar ATPase isoform VA68

Purification

Purified by antigen affinity column.

Accession

P38606

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

V-type proton ATPase catalytic subunit A (ATP6V1A) is a ~68 kDa protein. Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.

1. van Hille, B. et al. (1993) The Journal of biological chemistry 268, 7075-80. PMID: 8463241

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

4. Fassio, A. et al. (2018) Brain : a journal of neurology 141, 1703-1718. PMID: 29668857

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ATP6V1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: U87 cell lysate
Lane 4: Daudi cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat brain lysate

References

Datasheet

Anti-Human ATP6V1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Overview

Images

References

Datasheet

SDS

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