Anti-Human TLE3 Polyclonal Antibody (HC382014)

Name: Anti-Human TLE3 Polyclonal Antibody
Brand: abinScience
SKU: HC382014
Price: 168 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HC382014

Description

Anti-Human TLE3 Polyclonal Antibody (HC382014) is a rabbit polyclonal antibody detecting TLE3 in ELISA, IHC, WB. Suitable for Danio rerio, Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TLE3 (Ser449-Tyr772).

Target

Transducin-like enhancer protein 3, KIAA1547, TLE3, Enhancer of split groucho-like protein 3, ESG3

Purification

Purified by antigen affinity column.

Accession

Q04726

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transducin-like enhancer protein 3 (TLE3) is a ~83 kDa protein. Transcriptional coregulator that binds to a number of transcription factors. Acts as a regulator of adipogenesis both by acting as a corepressor of Wnt signaling and coactivator for PPARG. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. Promotes diffentiation of white adipocytes by acting as a coactivator for PPARG and preventing the association between PRDM16 and PPARG, thereby preventing browning of white adipocytes.

1. Flack, JE. et al. (2017) Molecular cell 67, 181-193.e5. PMID: 28689657

Note

For research use only.

Images

References

Datasheet

Anti-Human TLE3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TLE3 Polyclonal Antibody (HC382014)

Overview

Images

References

Datasheet

SDS

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