Anti-Human TPI1 Polyclonal Antibody (HX910014)

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Overview
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Datasheet

Overview

Catalog No.

HX910014

Description

Anti-Human TPI1 Polyclonal Antibody (HX910014) is a rabbit polyclonal antibody detecting TPI1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Dog, Chicken, Gorilla gorilla gorilla, Macaca fascicularis, Macaca mulatta, Mesocricetus auratus, Oryctolagus cuniculus, Pan troglodytes, Pongo abelii, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TPI1 (Met1-Gln249).

Target

TIM, Triose-phosphate isomerase, TPI, Methylglyoxal synthase, Triosephosphate isomerase, TPI1

Purification

Purified by antigen affinity column.

Accession

P60174

RRID

Anti-Human TPI1 Polyclonal Antibody (abinScience Cat# HX910014, RRID:AB_3730597)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Triosephosphate isomerase (TPI1) is a ~26 kDa protein. Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TPI1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: 293T cell lysate
Lane 4: U87 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse liver lysate
Lane 10: Mouse kidney lysate
Lane11: Mouse skeletal muscle lysate
Lane12: Rat brain lysate

References

Datasheet

Anti-Human TPI1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TPI1 Polyclonal Antibody (HX910014)

Overview

Images

References

Datasheet

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