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Anti-Human CDC25A Polyclonal Antibody (HB069014)

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Overview
Catalog No.HB069014
Description
Anti-Human CDC25A Polyclonal Antibody (HB069014) is a rabbit polyclonal antibody detecting CDC25A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CDC25A (Asp356-Leu524).
Target Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1, CDC25A
Purification Purified by antigen affinity column.
Accession P30304
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

M-phase inducer phosphatase 1 (CDC25A) is a ~59 kDa protein. Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin-E, in vitro.

1. Xiao, Z. et al. (2003) The Journal of biological chemistry 278, 21767-73. PMID: 12676925
2. Chen, MS. et al. (2003) Molecular and cellular biology 23, 7488-97. PMID: 14559997
3. Galaktionov, K. et al. (1991) Cell 67, 1181-94. PMID: 1836978
4. Timofeev, O. et al. (2010) The Journal of biological chemistry 285, 16978-90. PMID: 20360007
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CDC25A Polyclonal Antibody

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