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Anti-Human ATG14 Polyclonal Antibody (HP189014)

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Overview
Catalog No.HP189014
Description
Anti-Human ATG14 Polyclonal Antibody (HP189014) is a rabbit polyclonal antibody detecting ATG14 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ATG14 (Glu209-Glu415).
Target ATG14L, ATG14, Barkor, Atg14L, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, KIAA0831
Purification Purified by antigen affinity column.
Accession Q6ZNE5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Beclin 1-associated autophagy-related key regulator (ATG14) is a ~55 kDa protein. Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

1. Itakura, E. et al. (2008) Molecular biology of the cell 19, 5360-72. PMID: 18843052
2. Sun, Q. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 19211-6. PMID: 19050071
3. Matsunaga, K. et al. (2009) Nature cell biology 11, 385-96. PMID: 19270696
4. Matsunaga, K. et al. (2010) The Journal of cell biology 190, 511-21. PMID: 20713597
5. Fogel, AI. et al. (2013) Molecular and cellular biology 33, 3675-88. PMID: 23878393
6. Diao, J. et al. (2015) Nature 520, 563-6. PMID: 25686604
7. Shi, X. et al. (2023) Cell reports 42, 113045. PMID: 37632749
Note For research use only.
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Datasheet
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Anti-Human ATG14 Polyclonal Antibody

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