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Anti-Human CCT3 Polyclonal Antibody (HW312014)

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Overview
Catalog No.HW312014
Description
Anti-Human CCT3 Polyclonal Antibody (HW312014) is a rabbit polyclonal antibody detecting CCT3 in ELISA, IHC, WB. Suitable for Bovine, Human, Macaca fascicularis, Mouse, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CCT3 (Lys15-Lys527).
Target hTRiC5, CCT-gamma, T-complex protein 1 subunit gamma, TCP-1-gamma, CCTG, TRIC5, CCT3
Purification Purified by antigen affinity column.
Accession P49368
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

T-complex protein 1 subunit gamma (CCT3) is a ~60 kDa protein. Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.

1. Freund, A. et al. (2014) Cell 159, 1389-403. PMID: 25467444
2. Gestaut, D. et al. (2022) Cell 185, 4770-4787.e20. PMID: 36493755
3. Kelly, JJ. et al. (2022) Nature structural & molecular biology 29, 420-429. PMID: 35449234
4. Liu, C. et al. (2023) Communications biology 6, 531. PMID: 37193829
5. Seo, S. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 1488-93. PMID: 20080638
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human CCT3 Polyclonal Antibody

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