Anti-Human AGTPBP1 Polyclonal Antibody (HP163014)

Name: Anti-Human AGTPBP1 Polyclonal Antibody
Brand: abinScience
SKU: HP163014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HP163014

Description

Anti-Human AGTPBP1 Polyclonal Antibody (HP163014) is a rabbit polyclonal antibody detecting AGTPBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Chicken, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Chicken, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AGTPBP1 (Glu706-Cys1030).

Target

Cytosolic carboxypeptidase 1, Nervous system nuclear protein induced by axotomy protein 1 homolog, ATP/GTP-binding protein 1, CCP1, AGTPBP1, KIAA1035, NNA1

Purification

Purified by antigen affinity column.

Accession

Q9UPW5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytosolic carboxypeptidase 1 (AGTPBP1) is a ~138 kDa protein. Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of alpha-tubulin as well as non-tubulin proteins such as MYLK. Involved in KLF4 deglutamylation which promotes KLF4 proteasome-mediated degradation, thereby negatively regulating cell pluripotency maintenance and embryogenesis.

1. Berezniuk, I. et al. (2012) The Journal of biological chemistry 287, 6503-17. PMID: 22170066

2. Berezniuk, I. et al. (2013) The Journal of biological chemistry 288, 30445-30453. PMID: 24022482

3. Shashi, V. et al. (2018) The EMBO journal 37. PMID: 30420557

4. Ye, B. et al. (2018) Nature communications 9, 1261. PMID: 29593216

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human AGTPBP1 polyclonal antibody at 1ug/mL on recombinant Human AGTPBP1 (Catalog No. HP163012).

References

Datasheet

Anti-Human AGTPBP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human AGTPBP1 Polyclonal Antibody (HP163014)

Overview

Images

References

Datasheet

SDS

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