Anti-Human MARCHF8 Polyclonal Antibody (HP368014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HP368014

Description

Anti-Human MARCHF8 Polyclonal Antibody (HP368014) is a rabbit polyclonal antibody detecting MARCHF8 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Human, Mouse, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MARCHF8 (Ser13-Lys137).

Target

Cellular modulator of immune recognition, E3 ubiquitin-protein ligase MARCHF8, MARCH-VIII, MIR, RING finger protein 178, MARCHF8, Membrane-associated RING-CH protein VIII, c-MIR, MARCH8, Membrane-associated RING finger protein 8, RNF178, RING-type E3 ubiquitin transferase MARCHF8

Purification

Purified by antigen affinity column.

Accession

Q5T0T0

RRID

Anti-Human MARCHF8 Polyclonal Antibody (abinScience Cat# HP368014, RRID:AB_3730686)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase MARCHF8 is a ~32 kDa protein. E3 ubiquitin-protein ligase that plays several important roles in innate immunity and adaptive immunity. Mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. Possesses a very broad antiviral activity by specifically inactivating different viral fusion proteins. Targets and ubiquitinates cytoplasmic lysine residues of viral envelope glycoproteins with single transmembrane domains leading to their lysosomal degradation. Therefore, shows broad-spectrum inhibition against many viruses including retroviruses, rhabdoviruses, arenaviruses, sarbecoviruses or influenzaviruses.

1. Liu, X. et al. (2021) Nature communications 12, 4427. PMID: 34285233

2. Zhang, Y. et al. (2022) Microbiology spectrum 10, e0061821. PMID: 35019698

3. Yang, X. et al. (2022) Science signaling 15, eabk3067. PMID: 35503863

4. Lapaque, N. et al. (2009) The Journal of biological chemistry 284, 7007-16. PMID: 19117940

5. Gauvreau, ME. et al. (2009) Traffic (Copenhagen, Denmark) 10, 1518-27. PMID: 19566897

6. Yu, C. et al. (2020) mBio 11. PMID: 32934085

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MARCHF8 polyclonal antibody at 1ug/mL on recombinant Human MARCHF8 (Catalog No. HP368012).

References

Datasheet

Anti-Human MARCHF8 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MARCHF8 Polyclonal Antibody (HP368014)

Overview

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References

Datasheet

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