Anti-Human XRCC6 Polyclonal Antibody (HY120014)

Name: Anti-Human XRCC6 Polyclonal Antibody
Brand: abinScience
SKU: HY120014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY120014

Description

Anti-Human XRCC6 Polyclonal Antibody (HY120014) is a rabbit polyclonal antibody detecting XRCC6 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human XRCC6 (Asp36-Lys238).

Target

Lupus Ku autoantigen protein p70, 5'-dRP lyase Ku70, Thyroid-lupus autoantigen, XRCC6, CTC box-binding factor 75 kDa subunit, X-ray repair complementing defective repair in Chinese hamster cells 6, G22P1, X-ray repair cross-complementing protein 6, ATP-dependent DNA helicase 2 subunit 1, DNA repair protein XRCC6, TLAA, ATP-dependent DNA helicase II 70 kDa subunit, CTCBF, 70 kDa subunit of Ku antigen, Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, CTC75

Purification

Purified by antigen affinity column.

Accession

P12956

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair protein Ku70 (XRCC6) is a ~69 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC5 (Ku80), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs.

1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912

2. Liu, H. et al. (2010) Biochemical and biophysical research communications 397, 245-50. PMID: 20493174

3. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234

4. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203

5. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108

6. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123

7. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065

8. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055

9. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306

10. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-XRCC6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: K562 cell lysate
Lane 6: 293T cell lysate

References

Datasheet

Anti-Human XRCC6 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human XRCC6 Polyclonal Antibody (HY120014)

Overview

Images

References

Datasheet

SDS

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