Anti-Human USP13 Polyclonal Antibody (HP604014)

Name: Anti-Human USP13 Polyclonal Antibody
Brand: abinScience
SKU: HP604014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP604014

Description

Anti-Human USP13 Polyclonal Antibody (HP604014) is a rabbit polyclonal antibody detecting USP13 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human USP13 (Ser344-Ser863).

Target

Isopeptidase T-3, Deubiquitinating enzyme 13, ISOT-3, ISOT3, Ubiquitin-specific-processing protease 13, Ubiquitin carboxyl-terminal hydrolase 13, Ubiquitin thioesterase 13, USP13

Purification

Purified by antigen affinity column.

Accession

Q92995

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a ~97 kDa protein. Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy, endoplasmic reticulum-associated degradation (ERAD), cell cycle progression or DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Alternatively, forms with NEDD4 a deubiquitination complex, which subsequently stabilizes VPS34 to promote autophagy. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13.

1. Chen, M. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 9119-24. PMID: 21571647

2. Esposito, M. et al. (2020) Oncogene 39, 6009-6023. PMID: 32772043

3. Kim, W. et al. (2021) DNA repair 100, 103063. PMID: 33592542

4. Xie, W. et al. (2020) Cell reports 30, 2807-2819.e4. PMID: 32101753

5. Shin, J. et al. (2022) Molecular medicine (Cambridge, Mass.) 28, 164. PMID: 36585612

6. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278

7. Sun, H. et al. (2017) Nature communications 8, 15534. PMID: 28534493

Note

For research use only.

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References

Datasheet

Anti-Human USP13 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human USP13 Polyclonal Antibody (HP604014)

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