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Anti-Human SNAPIN Polyclonal Antibody (HF649014)

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Overview
Catalog No.HF649014
Description
Anti-Human SNAPIN Polyclonal Antibody (HF649014) is a rabbit polyclonal antibody detecting SNAPIN in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human SNAPIN (Gly21-Lys136).
Target Synaptosomal-associated protein 25-binding protein, SNAPIN, Biogenesis of lysosome-related organelles complex 1 subunit 7, SNARE-associated protein Snapin, BLOC-1 subunit 7, BLOC1S7, SNAPAP, SNAP25BP, SNAP-associated protein
Purification Purified by antigen affinity column.
Accession O95295
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

SNARE-associated protein Snapin (SNAPAP) is a ~14 kDa protein. Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25.

1. Setty, SR. et al. (2007) Molecular biology of the cell 18, 768-80. PMID: 17182842
2. Granata, A. et al. (2008) The Journal of biological chemistry 283, 7568-79. PMID: 18167355
3. Pu, J. et al. (2015) Developmental cell 33, 176-88. PMID: 25898167
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Anti-Human SNAPIN Polyclonal Antibody

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