Anti-Human RSAD2 Polyclonal Antibody (HP575014)

Name: Anti-Human RSAD2 Polyclonal Antibody
Brand: abinScience
SKU: HP575014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HP575014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RSAD2 (Ser74-Trp361).

Target

RSAD2, Viperin, Cytomegalovirus-induced gene 5 protein, Radical S-adenosyl methionine domain-containing protein 2, Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible, CIG5

Purification

Purified by antigen affinity column.

Accession

Q8WXG1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 is a ~42 kDa protein. Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1). Also promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6.

1. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 31812350

2. Gizzi, AS. et al. (2018) Nature 558, 610-614. PMID: 29925952

3. Dumbrepatil, AB. et al. (2019) The Journal of biological chemistry 294, 6888-6898. PMID: 30872404

4. Wei, C. et al. (2018) Viruses 11. PMID: 30587778

5. Vanwalscappel, B. et al. (2019) International journal of molecular sciences 20. PMID: 30934824

6. Li, M. et al. (2019) Frontiers in immunology 10, 2810. PMID: 31921110

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RSAD2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HUVEC cell lysate
Lane 2: Mouse spleen lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse lung lysate
Lane 5: Rat spleen lysate
Lane 6: Rat brain lysate
Lane 7: Rat lung lysate

References

Datasheet

Anti-Human RSAD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RSAD2 Polyclonal Antibody (HP575014)

Overview

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References

Datasheet

SDS

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