Anti-Human ATP6V0D1 Polyclonal Antibody (HX941014)

Name: Anti-Human ATP6V0D1 Polyclonal Antibody
Brand: abinScience
SKU: HX941014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX941014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATP6V0D1 (Met1-Phe351).

Target

V-ATPase AC39 subunit, V-ATPase subunit d 1, ATP6D, V-type proton ATPase subunit d 1, VPATPD, Vacuolar proton pump subunit d 1, ATP6V0D1, V-ATPase 40 kDa accessory protein, 32 kDa accessory protein, p39

Purification

Purified by antigen affinity column.

Accession

P61421

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

V-type proton ATPase subunit d 1 (ATP6V0D1) is a ~40 kDa protein. Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. May play a role in coupling of proton transport and ATP hydrolysis. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.

1. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

2. Jung, YS. et al. (2018) Nature cell biology 20, 1421-1433. PMID: 30374053

3. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

Note

For research use only.

Images

References

Datasheet

Anti-Human ATP6V0D1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP6V0D1 Polyclonal Antibody (HX941014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us