Anti-Human MCM3 Polyclonal Antibody (HB934014)

Name: Anti-Human MCM3 Polyclonal Antibody
Brand: abinScience
SKU: HB934014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB934014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MCM3 (Ser118-Lys656).

Target

p102, MCM3, DNA replication licensing factor MCM3, RLF subunit beta, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3

Purification

Purified by antigen affinity column.

Accession

P25205

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA replication licensing factor MCM3 is a ~90 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division (Probable).

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MCM3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate

References

Datasheet

Anti-Human MCM3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human MCM3 Polyclonal Antibody (HB934014)

Overview

Images

References

Datasheet

SDS

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