Anti-Human Quaking/QKI Polyclonal Antibody (HP249014)

Name: Anti-Human Quaking/QKI Polyclonal Antibody
Brand: abinScience
SKU: HP249014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP249014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human QKI (Thr14-Gly202).

Target

QKI, HqkI, HKQ, Hqk, Protein quaking

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

Q96PU8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

KH domain-containing RNA-binding protein QKI is a ~37 kDa protein. RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/or translation. Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, lipid deposition, interferon response, glial cell fate and development. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N(7)-methylguanine (m7G). Promotes the formation of circular RNAs (circRNAs) during the epithelial to mesenchymal transition and in cardiomyocytes: acts by binding to sites flanking circRNA-forming exons.

1. Fu, H. et al. (2012) Molecular biology of the cell 23, 1628-35. PMID: 22398723

2. Teplova, M. et al. (2013) Genes & development 27, 928-40. PMID: 23630077

3. Conn, SJ. et al. (2015) Cell 160, 1125-34. PMID: 25768908

4. de Bruin, RG. et al. (2016) Nature communications 7, 10846. PMID: 27029405

5. Caines, R. et al. (2019) Journal of cell science 132. PMID: 31331967

6. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838

7. Liao, KC. et al. (2020) RNA biology 17, 366-380. PMID: 31829086

8. Liao, KC. et al. (2021) Nucleic acids research 49, 10034-10045. PMID: 34428287

Note

For research use only.

Images

References

Datasheet

Anti-Human Quaking/QKI Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human Quaking/QKI Polyclonal Antibody (HP249014)

Overview

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Datasheet

SDS

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