Anti-Human U2AF1 Polyclonal Antibody (HX009014)

Name: Anti-Human U2AF1 Polyclonal Antibody
Brand: abinScience
SKU: HX009014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX009014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human U2AF1 (Met1-Arg196).

Target

U2AFBP, U2 auxiliary factor 35 kDa subunit, U2AF1, U2 snRNP auxiliary factor small subunit, U2 small nuclear RNA auxiliary factor 1, Splicing factor U2AF 35 kDa subunit, U2AF35

Purification

Purified by antigen affinity column.

Accession

Q01081

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Splicing factor U2AF 35 kDa subunit (U2AF1) is a ~27 kDa protein. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-U2AF1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raji cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Mouse kidney lysate
Lane 6: Rat kidney lysate

References

Datasheet

Anti-Human U2AF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human U2AF1 Polyclonal Antibody (HX009014)

Overview

Images

References

Datasheet

SDS

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