Anti-Human AP2A1 Polyclonal Antibody (HF867014)

Name: Anti-Human AP2A1 Polyclonal Antibody
Brand: abinScience
SKU: HF867014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF867014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AP2A1 (Ile737-Phe977).

Target

AP-2 complex subunit alpha-1, Alpha1-adaptin, Adaptor protein complex AP-2 subunit alpha-1, 100 kDa coated vesicle protein A, Clathrin assembly protein complex 2 alpha-A large chain, ADTAA, CLAPA1, AP2A1, Adaptor-related protein complex 2 subunit alpha-1, Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit, Alpha-adaptin A

Purification

Purified by antigen affinity column.

Accession

O95782

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

AP-2 complex subunit alpha-1 (AP2A1) is a ~107 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AP2A1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: SKOV3 cell lysate
Lane 2: THP-1 cell lysate

References

Datasheet

Anti-Human AP2A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human AP2A1 Polyclonal Antibody (HF867014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us