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Anti-Human POLI Polyclonal Antibody (HP553014)

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Overview
Catalog No.HP553014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human POLI (Arg53-Lys439).
Target RAD30 homolog B, RAD30B, DNA polymerase iota, Eta2, POLI
Purification Purified by antigen affinity column.
Accession Q9UNA4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA polymerase iota (POLI) is a ~83 kDa protein. Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.

1. Tissier, A. et al. (2000) The EMBO journal 19, 5259-66. PMID: 11013228
2. Frank, EG. et al. (2001) The EMBO journal 20, 2914-22. PMID: 11387224
3. Haracska, L. et al. (2003) Genes & development 17, 2777-85. PMID: 14630940
4. Washington, MT. et al. (2004) Molecular and cellular biology 24, 5687-93. PMID: 15199127
5. Zhou, Y. et al. (2010) Nucleic acids research 38, 5036-46. PMID: 20388628
6. Nair, DT. et al. (2004) Nature 430, 377-80. PMID: 15254543
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human POLI Polyclonal Antibody

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