Anti-Human FEN1 Polyclonal Antibody (HW308014)

Name: Anti-Human FEN1 Polyclonal Antibody
Brand: abinScience
SKU: HW308014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW308014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human FEN1 (Met1-Thr336).

Target

FEN-1, Maturation factor 1, hFEN-1, DNase IV, RAD2, FEN1, Flap endonuclease 1, MF1, Flap structure-specific endonuclease 1

Purification

Purified by antigen affinity column.

Accession

P39748

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Flap endonuclease 1 (FEN1) is a ~42 kDa protein. Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FEN1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Daudi cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: Jurkat cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Mouse testis lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat testis lysate
Lane 10: Rat spleen lysate

References

Datasheet

Anti-Human FEN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human FEN1 Polyclonal Antibody (HW308014)

Overview

Images

References

Datasheet

SDS

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