Anti-Human GNAT1 Polyclonal Antibody (HY277014)

Name: Anti-Human GNAT1 Polyclonal Antibody
Brand: abinScience
SKU: HY277014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HY277014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GNAT1 (Met1-Phe350).

Target

Transducin alpha-1 chain, GNATR, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNAT1

Purification

Purified by antigen affinity column.

Accession

P11488

RRID

Anti-Human GNAT1 Polyclonal Antibody (abinScience Cat# HY277014, RRID:AB_3730917)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Guanine nucleotide-binding protein G(t) subunit alpha-1 (GNAT1) is a ~40 kDa protein. Functions as a signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding.

1. Naeem, MA. et al. (2012) Investigative ophthalmology & visual science 53, 1353-61. PMID: 22190596

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human GNAT1 polyclonal antibody at 1ug/mL on recombinant Human GNAT1 (Catalog No. HY277012).

References

Datasheet

Anti-Human GNAT1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GNAT1 Polyclonal Antibody (HY277014)

Overview

Images

References

Datasheet

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