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Anti-Human GLO1 Polyclonal Antibody (HC529014)

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Overview
Catalog No.HC529014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human GLO1 (Gln33-Met184).
Target Glyoxalase I, Glx I, Ketone-aldehyde mutase, GLO1, Lactoylglutathione lyase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, Aldoketomutase
Purification Purified by antigen affinity column.
Accession Q04760
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lactoylglutathione lyase (GLO1) is a ~20 kDa protein. Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.

1. Birkenmeier, G. et al. (2010) PloS one 5, e10399. PMID: 20454679
2. Chiba, T. et al. (2012) Bioorganic & medicinal chemistry letters 22, 7486-9. PMID: 23122816
3. Ridderström, M. et al. (1998) The Journal of biological chemistry 273, 21623-8. PMID: 9705294
4. de Hemptinne, V. et al. (2009) Molecular and cellular biochemistry 325, 169-78. PMID: 19199007
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human GLO1 Polyclonal Antibody

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