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Anti-Human GPAT3 Polyclonal Antibody (HS846014)

Anti-Human GPAT3 Polyclonal Antibody
Anti-Human GPAT3 Polyclonal Antibody
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Overview
Catalog No.HS846014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GPAT3 (Ser207-Ser434).
Target 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, AGPAT 10, 1-AGPAT 9, GPAT-3, hGPAT3, Glycerol-3-phosphate acyltransferase 3, Lysophosphatidic acid acyltransferase theta, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10, LPAAT-theta, Acyl-CoA:glycerol-3-phosphate acyltransferase 3, 1-AGP acyltransferase 9, Lung cancer metastasis-associated protein 1, MAG1, MAG-1, GPAT3, AGPAT9
Purification Purified by antigen affinity column.
Accession Q53EU6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycerol-3-phosphate acyltransferase 3 (GPAT3) is a ~48 kDa protein. Converts glycerol-3-phosphate to 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) by incorporating an acyl moiety at the sn-1 position of the glycerol backbone. Also converts LPA into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Protects cells against lipotoxicity.

1. Cao, J. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 19695-700. PMID: 17170135
2. Sukumaran, S. et al. (2009) Journal of molecular endocrinology 42, 469-78. PMID: 19318427
3. Piccolis, M. et al. (2019) Molecular cell 74, 32-44.e8. PMID: 30846318
Note For research use only.
Images
  • Anti-Human GPAT3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human GPAT3 polyclonal antibody at 1ug/mL on recombinant Human GPAT3 (Catalog No. HS846012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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