Anti-Human TSC2 Polyclonal Antibody (HW726014)

Name: Anti-Human TSC2 Polyclonal Antibody
Brand: abinScience
SKU: HW726014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HW726014

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TSC2 (Asp1541-Asn1731).

Target

Tuberous sclerosis 2 protein, TSC2, TSC4, Tuberin

Purification

Purified by antigen affinity column.

Accession

P49815

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tuberin (TSC2) is a ~200 kDa protein. Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

5. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

6. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

7. Menon, S. et al. (2014) Cell 156, 771-85. PMID: 24529379

8. Lim, JS. et al. (2017) American journal of human genetics 100, 454-472. PMID: 28215400

9. Yang, H. et al. (2021) Nature communications 12, 339. PMID: 33436626

10. Ali, ES. et al. (2022) Molecular cell 82, 3284-3298.e7. PMID: 35772404

Note

For research use only.

Images

References

Datasheet

Anti-Human TSC2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human TSC2 Polyclonal Antibody (HW726014)

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Datasheet

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