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Anti-Human PRKG2 Polyclonal Antibody (HC398014)

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Overview
Catalog No.HC398014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PRKG2 (Ser431-Phe762).
Target cGK2, cGK 2, PRKG2, cGKII, PRKGR2, cGMP-dependent protein kinase II, cGMP-dependent protein kinase 2
Purification Purified by antigen affinity column.
Accession Q13237
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

cGMP-dependent protein kinase 2 (PRKG2) is a ~87 kDa protein. Crucial regulator of intestinal secretion and bone growth. Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum. Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863.

1. Díaz-González, F. et al. (2022) Journal of medical genetics 59, 28-38. PMID: 33106379
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human PRKG2 Polyclonal Antibody

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