Anti-Human RBM10 Polyclonal Antibody (HX074014)

Name: Anti-Human RBM10 Polyclonal Antibody
Brand: abinScience
SKU: HX074014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HX074014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RBM10 (Ser128-Ser207).

Target

GPATC9, S1-1, G patch domain-containing protein 9, RNA-binding protein S1-1, RNA-binding protein 10, GPATCH9, RBM10, RNA-binding motif protein 10, DXS8237E, KIAA0122

Purification

Purified by antigen affinity column.

Accession

P98175

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA-binding protein 10 (RBM10) is a ~103 kDa protein. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A). May bind to specific miRNA hairpins.

1. Nguyen, CD. et al. (2011) Journal of molecular biology 407, 273-83. PMID: 21256132

2. Inoue, A. et al. (2008) Biology of the cell 100, 523-35. PMID: 18315527

3. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RBM10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate

References

Datasheet

Anti-Human RBM10 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RBM10 Polyclonal Antibody (HX074014)

Overview

Images

References

Datasheet

SDS

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