Anti-Human VHL Polyclonal Antibody (HW527014)

Name: Anti-Human VHL Polyclonal Antibody
Brand: abinScience
SKU: HW527014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW527014

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VHL (Glu70-Asp213).

Target

VHL, pVHL, Protein G7, von Hippel-Lindau disease tumor suppressor

Purification

Purified by antigen affinity column.

Accession

P40337

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

von Hippel-Lindau disease tumor suppressor (VHL) is a ~24 kDa protein. Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.

1. Tanimoto, K. et al. (2000) The EMBO journal 19, 4298-309. PMID: 10944113

2. Cheng, J. et al. (2007) Cell 131, 584-95. PMID: 17981124

3. Xie, L. et al. (2009) Science signaling 2, ra33. PMID: 19584355

4. Ganner, A. et al. (2021) Scientific reports 11, 14827. PMID: 34290272

Note

For research use only.

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References

Datasheet