Anti-Human DHX15 Polyclonal Antibody (HT458014)

Name: Anti-Human DHX15 Polyclonal Antibody
Brand: abinScience
SKU: HT458014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HT458014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DHX15 (Asp568-Tyr795).

Target

DDX15, DEAH box protein 15, Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, DHX15, DBP1, ATP-dependent RNA helicase #46

Purification

Purified by antigen affinity column.

Accession

O43143

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-dependent RNA helicase DHX15 is a ~90 kDa protein. RNA helicase involved in mRNA processing and antiviral innate immunity. Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome. Acts as an RNA virus sensor: recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3).

1. Yoshimoto, R. et al. (2009) Nucleic acids research 37, 891-902. PMID: 19103666

2. Lin, ML. et al. (2009) Cancer science 100, 1443-50. PMID: 19432882

3. Mosallanejad, K. et al. (2014) Science signaling 7, ra40. PMID: 24782566

4. Lu, H. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 1364-72. PMID: 24990078

5. Studer, MK. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 7159-7170. PMID: 32179686

6. Xing, J. et al. (2021) Cell reports 35, 109205. PMID: 34161762

Note

For research use only.

Images

References

Datasheet

Anti-Human DHX15 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human DHX15 Polyclonal Antibody (HT458014)

Overview

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References

Datasheet

SDS

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