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Anti-Human ALDH4A1 Polyclonal Antibody (HB790014)

Anti-Human ALDH4A1 Polyclonal Antibody
Anti-Human ALDH4A1 Polyclonal Antibody
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  • Overview

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Overview
Catalog No.HB790014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ALDH4A1 (Ser44-Gln563).
Target ALDH4, ALDH4A1, L-glutamate gamma-semialdehyde dehydrogenase, Aldehyde dehydrogenase family 4 member A1, P5CDH, P5C dehydrogenase, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Purification Purified by antigen affinity column.
Accession P30038
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (ALDH4A1) is a ~61 kDa protein. Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Note For research use only.
Images
  • Anti-Human ALDH4A1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ALDH4A1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse kidney lysate
    Lane 3: Rat liver lysate
    Lane 4: Rat kidney lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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