Anti-Human DIABLO Polyclonal Antibody (HP429014)

Name: Anti-Human DIABLO Polyclonal Antibody
Brand: abinScience
SKU: HP429014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HP429014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DIABLO (His66-Asp239).

Target

Second mitochondria-derived activator of caspase, Diablo homolog, mitochondrial, DIABLO, Smac, SMAC, Direct IAP-binding protein with low pI

Purification

Purified by antigen affinity column.

Accession

Q9NR28

RRID

Anti-Human DIABLO Polyclonal Antibody (abinScience Cat# HP429014, RRID:AB_3731249)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Diablo IAP-binding mitochondrial protein is a ~27 kDa protein. Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases.

1. Bartke, T. et al. (2004) Molecular cell 14, 801-11. PMID: 15200957

2. Hunkeler, M. et al. (2023) Science (New York, N.Y.) 379, 1105-1111. PMID: 36758104

3. Ehrmann, JF. et al. (2023) Science (New York, N.Y.) 379, 1117-1123. PMID: 36758105

4. Dietz, L. et al. (2023) Science (New York, N.Y.) 379, 1112-1117. PMID: 36758106

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human DIABLO polyclonal antibody at 1ug/mL on recombinant Human DIABLO (Catalog No. HP429012).

References

Datasheet

Anti-Human DIABLO Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human DIABLO Polyclonal Antibody (HP429014)

Overview

Images

References

Datasheet

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