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Anti-Human NMT1 Polyclonal Antibody (HB608014)

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Overview
Catalog No.HB608014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NMT1 (Thr108-Gln496).
Target Myristoyl-CoA:protein N-myristoyltransferase 1, Type I N-myristoyltransferase, NMT 1, Peptide N-myristoyltransferase 1, NMT1, Glycylpeptide N-tetradecanoyltransferase 1, NMT
Purification Purified by antigen affinity column.
Accession P30419
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycylpeptide N-tetradecanoyltransferase 1 (NMT1) is a ~56 kDa protein. Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3'. Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle.

1. Montgomery, C. et al. (2012) The Journal of biological chemistry 287, 33227-36. PMID: 22865860
2. Thinon, E. et al. (2014) Nature communications 5, 4919. PMID: 25255805
3. Chen, YC. et al. (2020) Scientific reports 10, 11952. PMID: 32686708
4. Sun, Y. et al. (2022) Cancer letters 529, 126-138. PMID: 34999170
5. Glover, CJ. et al. (1997) The Journal of biological chemistry 272, 28680-9. PMID: 9353336
6. Giang, DK. et al. (1998) The Journal of biological chemistry 273, 6595-8. PMID: 9506952
7. Kosciuk, T. et al. (2020) Nature communications 11, 1067. PMID: 32103017
8. Dian, C. et al. (2020) Nature communications 11, 1132. PMID: 32111831
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human NMT1 Polyclonal Antibody

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