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Anti-Human ARPC1B Polyclonal Antibody (HT240014)

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Overview
Catalog No.HT240014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ARPC1B (Met1-Lys372).
Target Actin-related protein 2/3 complex subunit 1B, p41-ARC, Arp2/3 complex 41 kDa subunit, ARC41, ARPC1B
Purification Purified by antigen affinity column.
Accession O15143
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Actin-related protein 2/3 complex subunit 1B (ARPC1B) is a ~40 kDa protein. Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

1. Gournier, H. et al. (2001) Molecular cell 8, 1041-52. PMID: 11741539
2. Welch, MD. et al. (1997) The Journal of cell biology 138, 375-84. PMID: 9230079
3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ARPC1B Polyclonal Antibody

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