Please ensure Javascript is enabled for purposes of website accessibility
Contact us Contact Us
EN
Home > Products > Antibodies > Primary antibodies

Anti-Human LIMK1 Polyclonal Antibody (HW511014)

Datasheet Share product Copy info
Price(USD):
Spec:
  • 50ug
  • 100ug
Number:
Get quote

  • Overview

  • Images

  • References

  • Datasheet

  • SDS

Overview
Catalog No.HW511014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human LIMK1 (Gly419-Asp647).
Target LIMK-1, LIMK, LIMK1, LIM domain kinase 1
Purification Purified by antigen affinity column.
Accession P53667
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159
2. Niwa, R. et al. (2002) Cell 108, 233-46. PMID: 11832213
3. Kaji, N. et al. (2003) The Journal of biological chemistry 278, 33450-5. PMID: 12807904
4. Soosairajah, J. et al. (2005) The EMBO journal 24, 473-86. PMID: 15660133
5. Nishita, M. et al. (2005) The Journal of cell biology 171, 349-59. PMID: 16230460
6. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908
7. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514
8. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677
Note For research use only.
Images
References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: [email protected]

Distributor list

Get a free quote
Anti-Human LIMK1 Polyclonal Antibody

Quick access

Contact Us

  • +86-27-65523339

  • [email protected]

  • Building C, No. 666, Shen Dun Si Lu, Wuhan, 430206, China

  • linkedin
  • Ins
  • youtube
  • youtube
Copyright © 2026 abinScience. All Rights Reserved. All Products are for Research Use Only